Acetylation of Prostaglandin Endoperoxide Synthetase with Acetylsalicylic Acid

Inhibition of prostaglandin endoperoxide synthetase by thiol analogues of prostaglandin.

A variety of thiol compounds inhibited the enzymatic bis-oxygenation of 8,11,14-eicosatrienoic acid to prostaglandin G1, as examined with a purified preparation of prostaglandin endoperoxide synthetase (prostaglandin synthase; 8,11,14-eicosatrienoate, hydrogen-donor:oxygen oxidoreductase; EC 1.14.99.1) from bovine vesicular gland. The hydroperoxide cleavage of prostaglandin G1 producing prostag...

Prostaglandin endoperoxide synthetase isoenzymes: the clinical relevance of selective inhibition.

Purification of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes.

The prostaglandin synthetase system of bovine vesicular gland microsomes was solubilized and separated into Fractions I and II. The former fraction catalyzed the conversion of 8,11,14-eicosatrienoic acid to prostaglandin H1 (9 alpha, 11alpha-epidioxy-15(S)-hydroxy-13-trans-prostenoic acid). This compound was isomerized to prostaglandin E1 (11alpha, 15(S)-dihydroxy-9-keto-13-trans-prostenoic aci...

Prostaglandin hydroperoxidase, an integral part of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes.

The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group, producing prostaglandin H1. The latter enzymatic reaction required heme and was s...

The oxidation of 2-aminofluorene by prostaglandin endoperoxide synthetase. Comparison with other peroxidases.

We have examined the prostaglandin endoperoxide synthetase-dependent metabolism of the arylamine carcinogen 2-aminofluorene (2-AF). Ram seminal vesicle microsomes fortified with arachidonic acid metabolize 2-AF to products covalently bound to microsomal macromolecules, water-soluble metabolites, and two organic extractable metabolites. The organic extractable metabolites were identified by co-c...